About this position
- Postdoctoral Fellow: Protein Engineering
- The Division of Pulmonary, Critical Care and Sleep Medicine at the
- University of Maryland, Baltimore, School of Medicine is seeking a
- highly motivated Postdoctoral Researcher to engineer proteins with
- novel or improved functions using directed evolution and rational
- design, and to develop high-throughput selection and screening
- platforms that support an iterative design-build-test cycle across our
- protein engineering, synthetic biology, and molecular imaging
- programs.
Key Responsibilities
- Design and construct combinatorial, site-saturation, and rationally designed variant libraries.
- Develop and execute high-throughput selection and screening strategies, including growth-coupled selections and FACS-based screens.
- Express, purify, and biophysically characterize engineered proteins, including binding, stability, and specificity measurements.
- Integrate validated variants into cellular and in vivo systems and confirm function in a biological context.
- Generate high-throughput sequencing datasets from selection campaigns and work with computational colleagues to implement and test their designs.
- Contribute protein biochemistry expertise across the lab’s broader research program as needed.
- Present findings at internal meetings and scientific conferences and prepare manuscripts for highimpact journals.
- Mentor junior researchers and contribute to a collaborative lab environment.
Qualifications
- PhD in Biochemistry, Protein Engineering, Molecular Biology, Chemical Biology, or a related field.
- Strong hands-on experience in protein engineering, including library construction, directed evolution or high-throughput selection, and protein expression and purification.
- Proficiency in molecular biology techniques, including cloning and synthetic construct design, PCRbased mutagenesis, and next-generation sequencing library preparation.
- Experience with biophysical characterization methods for protein-ligand and protein-protein interactions.
- Experience with deep mutational scanning or display technologies (phage, yeast, or bacterial surface display) is desirable.
- Experience with membrane protein expression, structural biology, or bacterial genome engineering is a plus.
- Ability to work independently and within a collaborative team; strong communication and writing skills.
- Application: Interested candidates should send their CV, a cover
- letter outlining their research experience, interest in this position
- and contact information for three references to Dr. Nalinikanth
- Kotagiri at NKotagiri@som.umaryland.edu
Desired start date: Immediately
How to apply
- Read the full advert on the source site — it carries the authoritative terms.
- Email the contact below with your CV and a short, specific message. See Emailing Professors.
- Prepare your SOP, CV, transcripts and referees before the deadline.
- Apply through the university's own portal. Never pay a fee to a third party.