About this position
About the Project
This project has received funding from the European Research Council (ERC) under the European Union's Horizon Europe research and innovation programme (Grant Agreement No.101265901)
The T6S-PoreMech project, funded by a prestigious ERC Advanced Grant, aims to establish a new unifying framework for understanding Type VI Secretion System (T6SS) Pore-Forming Toxins (PFTs). By combining cutting-edge single-particle cryo-electron microscopy (cryo-EM), membrane protein biochemistry, and single-channel electrophysiology, the project will resolve the atomic architectures of active, membrane-inserted toxin pores and decipher how bacteria defend themselves against these molecular weapons.
We are seeking three highly motivated and talented team members to join this exciting 5-year initiative at Instituto Biofisika:
Open Positions
- Postdoctoral Researcher – Biophysics & Toxin Function (Postdoc 1)
? Role: Lead the in vivo and in vitro functional characterization of T6SS pore-forming toxins and their biophysical molecular mechanisms.
? Essential qualifications:
o Ph.D. in Biophysics, Biochemistry, Molecular Biology, or a related field.
o Demonstrated expertise in biophysics, membrane proteins, and/or pore-forming toxins.
? Desirable qualifications:
o Experience with single-channel planar lipid bilayer electrophysiology, liposome/nanodisc reconstitutions, or flow cytometry-based cellular assays.
- Postdoctoral Researcher – Cryo-EM & Structural Biology (Postdoc 2)
? Role: Drive high-resolution single-particle cryo-EM structure determination of active, membrane-inserted toxin pores in nanodiscs and toxin-immunity complexes using megabody technologies.
? Essential qualifications:
o Ph.D. in Structural Biology, Biochemistry, Biophysics, or a related field.
o Demonstrated expertise in cryo-EM (sample preparation, grid vitrification, data collection, and processing), membrane proteins, or pore-forming toxins.
? Desirable qualifications:
o Experience with nanodisc reconstitution, megabodies/nanobodies, or membrane protein purification.
- Technical Specialist – Protein Biochemistry
? Role: Support the day-to-day laboratory operations, manage molecular biology pipelines, and lead the expression and purification of challenging bacterial membrane proteins to support all project pillars.
? Essential qualifications:
o B.Sc., M.Sc., or Ph.D. in Biochemistry, Biotechnology, or a related field.
o Proven expertise in protein biochemistry, specifically protein expression and purification (bacterial expression systems, affinity/size-exclusion chromatography).
? Desirable qualifications:
o Prior experience in handling membrane-associated proteins, lab management, and inventory ordering is highly valued.
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