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PhD call in biology: Drivers of polyploid genome evolution

University of Silesia in Katowice · Poland

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About this position

Subject : „Drivers of polyploid genome evolution: repetitive DNA dynamics and chromosome rearrangements ”

Unit of the project realization : Faculty of Natural Sciences; Institute of Biology, Biotechnology and Environmental Protection – University of Silesia in Katowice

Unit realizing the PhD student education: Doctoral School at the University of Silesia in Katowice

The project will focus on rDNA evolution and organization in Amaranthus using molecular, cytogenetic, bioinformatic, and phylogenetic approaches to understand post-polyploidization genome evolution, including sequence variation and chromosomal localization. It also investigates karyotype evolution and chromosome number changes, including chromosome fusions and dysploidy, using oligo-labeling probes, fluorescent in situ hybridisation, and comparative cytogenetic analyses.

Research will be carried out in the frame of the project „Drivers of polyploid genome evolution: repetitive DNA dynamics and chromosome rearrangements” (number of the project 2025/59/B/NZ8/00852 ), financed from NCN under management of dr hab. Bożena Kolano, prof. UŚ

Scope of Duties

  • Plant material cultivation and preparation
  • Chromosome number analyses and detection of inter- and intraspecific variation
  • DNA isolation and cloning of rDNA sequences
  • Preparation of chromosome spreads (including 3D polyacrylamide-based preparations)
  • Fluorescence in situ hybridization (FISH) and immunostaining
  • Epifluorescence and confocal microscopy analyses, including image processing
  • Participation in bioinformatic analyses
  • Design and application of oligonucleotide probes for FISH
  • Participation in data analysis, manuscript preparation, and conference presentation

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